phosphorylated stress‐activated protein kinase (sapk)/jnk  (Cell Signaling Technology Inc)

Journal: Arthritis Research & Therapy

Article Title: NLRP3 inflammasome inhibitor OLT1177 suppresses joint inflammation in murine models of acute arthritis

doi: 10.1186/s13075-018-1664-2

Figure Lengend Snippet: Effect of posttreatment of oral OLT1177 in monosodium urate-induced arthritis. Total white blood cell (WBC) count ( a ) and differential counts ( b ) in mice treated with vehicle or OLT1177 (600 mg/kg) ( n = 5). c Mean ± SEM of spontaneous (Spnt) interleukin (IL)-6 levels from whole-blood culture in vehicle- and OLT1177-treated mice. d Mean ± SEM of joint score ( n = 20). e–g Mean ± SEM of IL-1β, IL-6, and chemokine (C-X-C motif) ligand 1 (CXCL1) in synovial tissue extracts ( n = 10 per group). h Western blot of phospho-c-Jun N-terminal kinase (JNK) and IL-1β (p17 and IL-1β precursor p37) in synovial tissue extracts. Each lane represents a single mouse. **** p < 0.0001, ** p < 0.01, * p < 0.05. SAPK Stress-activated protein kinase

Article Snippet: Phosphorylated stress-activated protein kinase/c-Jun N-terminal kinase (JNK) (1:500; Cell Signaling Technology, Danvers, MA, USA), IL-1β (1:1000, AF-401; R&D Systems), and NLRP3 (1:1000, Cryo-2; AdipoGen Life Sciences, San Diego, CA, USA) were used as the primary antibodies.

Techniques: Western Blot